Product Pathways - Metabolism
Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit #12704
|12704S||1 Kit (4 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||MW (kDa)||Isotype|
|Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb #11818||40 µl||W, IP, IHC-P, IF-IC||H, M||280||Rabbit IgG|
|Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676||40 µl||W, IHC-P, IF-IC, F||H, M, R, Hm||280||Rabbit IgG|
|Acetyl-CoA Carboxylase 1 Antibody #4190||40 µl||W, IP, IF-IC||H, M, R||265||Rabbit|
|Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb #8578||40 µl||W, IP||H||280||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster
Western blot analysis of extracts from SH-SY5Y cells, untreated (-) or treated with Oligomycin #9996 (0.5 μM, 30 min; +), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb #11818 (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment.
Western blot analysis of cell extracts from various cell lines using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676.
Western blot analysis of extracts from various cell lines using Acetyl-CoA Carboxylase 1 Antibody #4190.
The Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit provides an economical means of distinguishing between the two acetyl-CoA carboxylase isoforms, and between total acetyl-CoA carboxylase and phosphorylated acetyl-CoA carboxylase. The kit includes enough antibody to perform four western blot experiments per primary antibody.
Specificity / Sensitivity
Each antibody recognizes endogenous levels of their target protein. Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human acetyl-CoA carboxylase 1 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein, or a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1, or Val1416 of human acetyl-CoA carboxylase 2 protein.
Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.