Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

MERIT40 (D7Y5K) Rabbit mAb #12711

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 40 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MERIT40 (D7Y5K) Rabbit mAb recognizes endogenous levels of total MERIT40 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MERIT40 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MERIT40 (D7Y5K) Rabbit mAb.

IP

IP

Immunoprecipitation of MERIT40 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or MERIT40 (D7Y5K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MERIT40 (D7Y5K) Rabbit mAb. A light-chain specific secondary antibody was used.

Background

The breast cancer susceptibility gene, BRCA1, codes for an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of DNA damage response and DNA repair. BRCA1 forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for the regulation of BRCA1 function. The BRCA1-Rap80 complex (BRCA1 A complex), including Rap80, BRCC36, BRCC45, Abraxas, and MERIT40/NBA1, functions in G2/M phase checkpoint control (reviewed in 1,2).MERIT40/NBA1 localizes to sites of DNA damage and is required for the appropriate localization of BRCA1 in response to ionizing radiation, as well as maintenance of the BRCA1 A complex (3,4). Proteomics studies have identified Ser29 as a phosphorylated site on MERIT40/NBA1, and the significance of this phosphorylation is under investigation (5-9).

  1. Ohta, T. et al. (2011) FEBS Lett 585, 2836-44.
  2. Huen, M.S. et al. (2010) Nat Rev Mol Cell Biol 11, 138-48.
  3. Wang, B. et al. (2009) Genes Dev 23, 729-39.
  4. Shao, G. et al. (2009) Genes Dev 23, 740-54.
  5. Moritz, A. et al. (2010) Sci Signal 3, ra64.
  6. Rigbolt, K.T. et al. (2011) Sci Signal 4, rs3.
  7. Iliuk, A.B. et al. (2010) Mol Cell Proteomics 9, 2162-72.
  8. Wu, F. et al. (2010) Mol Cell Proteomics 9, 1616-32.
  9. Mayya, V. et al. (2009) Sci Signal 2, ra46.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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