Product Pathways - Jak/Stat Pathway
Phospho-TPOR (Tyr626) (D3H7B) Rabbit mAb #12712
|12712S||100 µl (10 western blots)||---||In Stock||---|
|12712||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Bovine.
Specificity / Sensitivity
Phospho-TPOR (Y626) (D3H7B) Rabbit mAb recognizes endogenous levels of TPOR protein only when phosphorylated at Tyr626. This antibody cross-reacts with a 30 kDa protein of unknown origin. This antibody may cross-react with some tyrosine-phosphorylated proteins including the EGF Receptor, ROS1, and FLT3.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr626 of human TPOR protein.
Western blot analysis of extracts from serum-starved BaF3 cells or serum-starved BaF3 cells stably expressing mouse thrombopoietin receptor (mTPOR), untreated (-) or treated with recombinant mouse thrombopoietin (mTPO; 100 ng/mL, 10 min; +), using Phospho-TPOR (Tyr626) (D3H7B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Thrombopoietin receptor (TPOR, c-Mpl) is a hematopoietic receptor that binds the growth factor, thrombopoietin (TPO), responsible for regulation of platelet production (1-3). Expression of TPOR by megakaryocytes is required for megakaryocyte growth and development (4). TPOR is also expressed by hematopoietic stem cells and is required for stem cell maintenance and expansion (5). Studies show that mice lacking either TPOR or TPO have severely reduced numbers of platelets and megakaryocytes as well as decreased numbers of other hematopoietic lineages (4,5). Binding of TPO to TPOR induces receptor dimerization that leads to phosphorylation and activation of the tyrosine kinase Jak2. Activated Jak2 associates with the cytoplasmic domain of TPOR (6,7) and phosphorylates TPOR at Tyr626 and Tyr631 (8). These phosphorylated tyrosine residues provide docking sites for downstream signaling molecules including Stat3, Stat5, Shc, and SHIP (7-9).
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- Drachman, J.G. and Kaushansky, K. (1997) Proc Natl Acad Sci U S A 94, 2350-5.
- Gurney, A.L. et al. (1995) Proc Natl Acad Sci U S A 92, 5292-6.
- Bacon, C.M. et al. (1995) FEBS Lett 370, 63-8.
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