Product Pathways - Adhesion
uPAR (D7X2N) Rabbit mAb #12713
|12713S||100 µl (10 western blots)||---||In Stock||---|
|12713||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
uPAR (D7X2N) Rabbit mAb recognizes endogenous levels of total uPAR protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg303 of human uPAR protein.
Western blot analysis of extracts from U-937 cells, untreated (-) or treated (+) with TPA #4174 (200 nM, 72 hr), using uPAR (D7X2N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
The human urokinase-type plasminogen activator receptor (uPAR) is a 55-65 kDa, highly glycosylated, GPI-anchored cell surface receptor (the deglycosylated protein is 35 kDa) (1-3). It is a central player in the plasminogen activation pathway. uPAR binds with high affinity to a serine protease urokinase-type plasminogen activator (uPA) and converts plasminogen to its active form plasmin in a spatially restricted manner on the cell surface (4). Plasmin further carries out the activation of uPA, which is inhibited by serpins, such as plasminogen activator inhibitors (5). Therefore, uPAR plays a key role in regulating extracellular proteolysis. In addition, uPAR plays an important role in regulating cell proliferation, adhesion and mobility (6,7). Research studies have shown that overexpression of uPAR is found in various cancer cells and tissues (8,9).
- Nielsen, L.S. et al. (1988) J Biol Chem 263, 2358-63.
- Behrendt, N. et al. (1990) J Biol Chem 265, 6453-60.
- Roldan, A.L. et al. (1990) EMBO J 9, 467-74.
- Ellis, V. et al. (1991) J Biol Chem 266, 12752-8.
- Ellis, V. et al. (1990) J Biol Chem 265, 9904-8.
- Liu, D. et al. (2002) Cancer Cell 1, 445-57.
- Waltz, D.A. et al. (1997) J Clin Invest 100, 58-67.
- Blasi, F. and Sidenius, N. (2010) FEBS Lett 584, 1923-30.
- Mazar, A.P. et al. (2011) Curr Pharm Des 17, 1970-8.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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