Product Pathways - Vesicle Trafficking
ER and Golgi-Associated Marker Proteins Antibody Sampler Kit #12718
|12718S||1 Kit (5 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Calnexin (C5C9) Rabbit mAb #2679||40 µl||W, IHC-P, IF-IC||H, Mk||90||Rabbit|
|ERp72 (D70D12) XP® Rabbit mAb #5033||40 µl||W, IF-IC, F||H, M, R, Mk||72||Rabbit IgG|
|PDI (C81H6) Rabbit mAb #3501||40 µl||W, IHC-P, IF-IC||H, M, R, Mk||57||Rabbit|
|RCAS1 (D2B6N) XP® Rabbit mAb #12290||40 µl||W, IP, IF-IC, F||H, M, R||Mk||32||Rabbit IgG|
|Syntaxin 6 (C34B2) Rabbit mAb #2869||40 µl||W, IP, IF-IC||H, M, R||32||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IP=Immunoprecipitation
Reactivity Key: H=Human, Mk=Monkey, M=Mouse, R=Rat
Western blot analysis of extracts from various cell lines using RCAS1 (D2B6N) XP® Rabbit mAb #12290.
Western blot analysis of extracts from PANC-1, Hep G2, and A-204 cells using Calnexin (C5C9) Rabbit mAb #2679.
Western blot analysis of extracts from A172, Neuro-2a, and PC-12 cells using Syntaxin 6 (C34B2) Rabbit mAb #2869.
Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb #3501.
The ER and Golgi-Associated Marker Proteins Antibody Sampler Kit contains reagents to examine proteins that help regulate protein folding and vesicle trafficking. This kit includes enough antibody to perform four western blot experiments with each primary antibody.
Specificity / Sensitivity
Each antibody will detect endogenous total levels of their target protein.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human calnexin, the sequence of human PDI, the residues surrounding Met279 of human ERp72 protein, the residues surrounding Gly147 of human RCAS1 protein, and the residues surrounding Tyr140 of mouse syntaxin 6 protein.
Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation, and folding. The ER contains a pool of molecular chaperones to help proteins fold properly. Calnexin is a calcium-binding, ER membrane protein that ensures proper protein folding by retaining newly synthesized glycoproteins within the ER l (1-3). The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (3). Many secretory proteins require the formation of intra- or inter-molecular disulfide bonds to reach their native conformation (4). Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of disulfide bonds during oxidative protein folding (5). The ER-protein Ero1 oxidizes PDI through disulfide exchange, which is followed by PDI-catalyzed disulfide bond formation in folding proteins (6). The ER stress protein 72 (ERp72) contains three thioredoxin homology domains and plays a role in the formation and isomerization of disulfide bonds (7,8).
The tumor-associated antigen RCAS1 negatively regulates cytotoxic T lymphocyte (CTL) cytolytic activity, which impacts vesicle formation, secretion, and protein glycosylation (9-12). Overexpression of RCAS1 impairs CTL cytolytic function by negatively regulating trans-Golgi to secretory lysosome protein trafficking, leading to a delay in ER to Golgi vesicle transport and mislocalization of ER quality control and glycosylation proteins. As a result, RCAS1 induces deposition of tumor-associated glycan antigens on the cell surface, which may contribute to tumor pathogenesis through the mediation of adhesion, invasion, and metastasis (13,14). Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (15,16) that is localized to the trans-Golgi and within endosomes. It regulates membrane trafficking by partnering with a variety of other SNARE proteins (17-19) and is involved in the regulation of GLUT4 trafficking, neutrophil exocytosis, and granule secretion (20-22).
- Rajagopalan, S. et al. (1994) Science 263, 387-90.
- Bergeron, J.J. et al. (1994) Trends Biochem Sci 19, 124-8.
- Williams, D.B. (2006) J Cell Sci 119, 615-23.
- Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
- Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
- Tu, B.P. and Weissman, J.S. (2004) J Cell Biol 164, 341-6.
- Mazzarella, RA et al. (1990) J Biol Chem 265(2), 1094-101.
- Satoh, M et al. (2005) Cell Stress Chaperones 10(4), 278-84
- Rüder, C. et al. (2009) J Clin Invest 119, 2184-203.
- Reimer, T.A. et al. (2005) BMC Cancer 5, 47.
- Wolf, J. et al. (2010) FASEB J 24, 4000-19.
- Engelsberg, A. et al. (2003) J Biol Chem 278, 22998-3007.
- Bock, J.B. et al. (2001) Nature 409, 839-41.
- Bock, J.B. et al. (1996) J Biol Chem 271, 17961-5.
- Wendler, F. and Tooze, S. (2001) Traffic 2, 606-11.
- Bock, J.B. et al. (1997) Mol Biol Cell 8, 1261-71.
- Mallard, F. et al. (2002) J Cell Biol 156, 653-64.
- Perera, H.K. et al. (2003) Mol Biol Cell 14, 2946-58.
- Shewan, A.M. et al. (2003) Mol Biol Cell 14, 973-86.
- Martín-Martín, B. et al. (2000) Blood 96, 2574-83.
- Wendler, F. et al. (2001) Mol Biol Cell 12, 1699-709.
- Kuliawat, R. et al. (2004) Mol Biol Cell 15, 1690-701.
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* Product-specific protocol.
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- 9999 Nonfat Dry Milk
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.