Product Pathways - Transcription Factors
Aiolos Antibody #12720
|12720S||100 µl (10 western blots)||---||In Stock||---|
|12720||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Monkey.
Specificity / Sensitivity
Aiolos Antibody recognizes endogenous levels of total Aiolos protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human Aiolos protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human Aiolos (hAiolos; +), using Aiolos Antibody.
Aiolos is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family members (1). Multiple Aiolos isoforms are generated through alternative splicing of the portion of the transcript encoding the amino-terminal zinc fingers (2). Aiolos is expressed by lymphoid tissues, with highest expression levels seen in mature B and T cells (1). The Ikaros family controls lymphocyte development by recruiting chromatin remodeling complexes to DNA (3). B cells from mice lacking Aiolos have a reduced threshold for activation, increased proliferation, elevated IgG and IgE, and develop B cell lymphomas (4). In T cells, Aiolos contributes to Th17 cell differentiation by suppressing IL-2 expression (5). Alterations in the Aiolos gene are observed in near haploid acute lymphoblastic leukemia, and the genetic locus containing Aiolos is linked to increased susceptibility to rheumatoid arthritis and systemic lupus erythematosus (6-8).
- Morgan, B. et al. (1997) EMBO J 16, 2004-13.
- Liippo, J. et al. (2001) Eur J Immunol 31, 3469-74.
- Kim, J. et al. (1999) Immunity 10, 345-55.
- Wang, J.H. et al. (1998) Immunity 9, 543-53.
- Quintana, F.J. et al. (2012) Nat Immunol 13, 770-7.
- Holmfeldt, L. et al. (2013) Nat Genet 45, 242-52.
- Kurreeman, F.A. et al. (2012) Am J Hum Genet 90, 524-32.
- Lessard, C.J. et al. (2012) Am J Hum Genet 90, 648-60.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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