Product Pathways - Translational Control
NXF1 (D5X4G) Rabbit mAb #12735
|12735S||100 µl (10 western blots)||---||In Stock||---|
|12735||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||70||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Pig, Guinea Pig.
Specificity / Sensitivity
NXF1 (D5X4G) Rabbit mAb recognizes endogenous levels of total NXF1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln211 of human NXF1 protein.
mRNA export is a process that is tightly coupled to mRNA splicing (1-4). Splicing and packaging of mRNAs in the form of an mRNA-protein complex (mRNP) leads to the recruitment of the mRNA export adaptor THOC4/ALY, via its interaction with the splicing factor UAP56, forming a large complex termed the transcription-export complex (TREX) (1,2,5). THOC4/ALY then directly interacts with NXF1/TAP, a part of the heterodimer that targets the mRNP to the nuclear pore complex, resulting in the shuttling of mRNP out of the nucleus and into the cytoplasm (1-3,6).
- Katahira, J. (2012) Biochim Biophys Acta 1819, 507-13.
- Siddiqui, N. and Borden, K.L. (2012) Wiley Interdiscip Rev RNA 3, 13-25.
- Reed, R. and Magni, K. (2001) Nat Cell Biol 3, E201-4.
- Masuda, S. et al. (2005) Genes Dev 19, 1512-7.
- Luo, M.L. et al. (2001) Nature 413, 644-7.
- Kang, Y. and Cullen, B.R. (1999) Genes Dev 13, 1126-39.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.