Product Pathways - NF-kB Signaling
Phospho-IRAK1 (Thr209) Antibody #12756
|12756S||100 µl (10 western blots)||---||In Stock||---|
|12756||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-IRAK1 (Thr209) Antibody recognizes endogenous levels of IRAK1 protein only when phosphorylated at Thr209. This antibody does not detect unmodified 75 kDa IRAK1. This antibody cross-reacts with a 35 kDa protein of unknown origin.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr209 of human IRAK1 protein.
Western blot analysis of extracts from serum-starved KARPAS-299 cells, untreated (-) or treated with Human Interleukin-1β (hIL-1β) #8900 (50 ng/ml, 15 min; +), using Phospho-IRAK1 (Thr209) Antibody (upper) or IRAK1 (D51G7) XP® Rabbit mAb #4504 (lower). Cell Line Source: Dr Abraham Karpas at the University of Cambridge.
Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).
Upon IL-1R/Toll-Like Receptor (TLR) ligation, IRAK1 and IRAK4 are rapidly recruited to the receptor by the adaptor MyD88 (4). IRAK1 is phosphorylated by IRAK4 at Thr209 and Thr387, followed by sequential autohyperphosphorylation in various domains (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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