Product Pathways - Metabolism
SHMT2 Antibody #12762
|12762S||100 µl (10 western blots)||---||In Stock||---|
|12762||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||52||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
SHMT2 Antibody recognizes endogenous levels of total SHMT2 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe441 of human SHMT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing either Myc/DDK-tagged full-length human SHMT1 (hSHMT1-Myc/DDK; +) or Myc/DDK-tagged full-length human SHMT2 (hSHMT2-Myc/DDK; +), using SHMT2 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxylmethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5, 10-methylene-THF (1, 2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.