Product Pathways - Growth Factors/Cytokines
TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (PE Conjugate) #12779
|12779S||100 µl (50 tests)||---||In Stock||---|
|12779||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: F=Flow Cytometry
Specificity / Sensitivity
TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) (PE Conjugate) recognizes endogenous levels of total TNF-α protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant mouse TNF-α protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated TNF-α (D2D4) XP® Rabbit mAb (Rodent Specific) #11948.
TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.