Product Pathways - Translational Control
XBP-1s (D2C1F) Rabbit mAb #12782
|12782S||100 µl (10 western blots)||---||In Stock||---|
|12782||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Mouse, Rat.
Specificity / Sensitivity
XBP-1s (D2C1F) Rabbit mAb recognizes endogenous levels of the total spliced isoform of XBP1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu64 of human XBP1 protein.
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with tunicamycin (2 μg/ml, 8 hr; +), using XBP-1s (D2C1F) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human XBP-1s (hXBP-1s-Myc/DDK; +), using XBP-1s (D2C1F) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Following protein synthesis, secretory, intra-organellar, and transmembrane proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. The accumulation of unfolded proteins within the ER triggers an adaptive mechanism known as the unfolded protein response (UPR) that counteracts compromised protein folding (1). The transmembrane serine/threonine kinase IRE1, originally identified in Saccharomyces cerevisiae, is a proximal sensor for the UPR that transmits the unfolded protein signal across the ER membrane (2-4). The human homolog IRE1α was later identified and is ubiquitously expressed in human tissues (5). Upon activation of the unfolded protein response, IRE1α splices X-box binding protein 1 (XBP-1) mRNA through an unconventional mechanism using its endoribonuclease activity (6). This reaction converts XBP-1 from an unspliced XBP-1u isoform to the spliced XBP-1s isoform, which is a potent transcriptional activator that induces expression of many UPR responsive genes (6).
- Kaufman, R.J. et al. (2002) Nat Rev Mol Cell Biol 3, 411-21.
- Nikawa, J. and Yamashita, S. (1992) Mol Microbiol 6, 1441-6.
- Cox, J.S. et al. (1993) Cell 73, 1197-206.
- Mori, K. et al. (1993) Cell 74, 743-56.
- Tirasophon, W. et al. (1998) Genes Dev 12, 1812-24.
- Lee, K. et al. (2002) Genes Dev 16, 452-66.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.