Product Pathways - Chromatin Regulation / Epigenetics
Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit #12784
|12784S||1 Kit (7 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|ESET (C1C12) Rabbit mAb #2196||40 µl||W, IP, IF-IC||H, Mk||180||Rabbit IgG|
|Fibrillarin (C13C3) Rabbit mAb #2639||40 µl||W, IF-IC||H, M, R, Mk||37||Rabbit IgG|
|Histone H2A.Z Antibody #2718||40 µl||W, IP, IF-IC||H, M, R, Mk, Z||C, X, B||14||Rabbit|
|Histone H3 (D1H2) XP® Rabbit mAb #4499||40 µl||W, IHC-P, IF-IC||H, M, R, Mk||Hm, C, Dm, X, Z, B||17||Rabbit IgG|
|Lamin A/C (4C11) Mouse mAb #4777||40 µl||W, IP, IHC-P, IF-F, IF-IC, F||H, M, R, Mk||74 (Lamin A), 63 (Lamin C)||Mouse IgG2a|
|LSD1 (C69G12) Rabbit mAb #2184||40 µl||W, IP, IHC-P, IHC-F, IF-IC||H, M, R, Mk||110||Rabbit IgG|
|NUP98 (C39A3) Rabbit mAb #2598||40 µl||W, IP, IF-IC||H, M, R, Mk||98||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Anti-mouse IgG, HRP-linked Antibody #7076||100 µl||Horse|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), F=Flow Cytometry, IHC-F=Immunohistochemistry (Frozen)
Reactivity Key: H=Human, Mk=Monkey, M=Mouse, R=Rat, Z=Zebrafish
Western blot analysis of extracts from various cell lines using LSD1 (C69G12) Rabbit mAb #2184.
Western blot analysis of extracts from MCF7 and 293 cells using ESET (C1C12) Rabbit mAb #2196.
Western blot analysis of extracts from various cell lines using NUP98 (C39A3) Rabbit mAb #2598.
Western blot analysis of extracts from various cell lines using Fibrillarin (C13C3) Antibody #2639.
Western blot analysis of extracts from various cell lines, in addition to 10 ng of recombinant H2A (rH2A) and H2A.Z (rH2A.Z) protein, using Histone H2A.Z Antibody #2718 (upper) and Histone H2A Antibody II #2578 (lower).
Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb #4499.
The Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit provides an economical means to evaluate relevant nuclear proteins. This kit contains enough primary antibody to perform at least four western blots per primary antibody.
Specificity / Sensitivity
Each antibody in the Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit recognizes total endogenous levels of the specific target protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human ESET protein, residues surrounding Thr298 of human fibrillarin, the carboxy terminus of the human histone H3 protein, a recombinant fragment of human lamin A protein, residues near the amino-terminus of human LSD1 protein, or residues surrounding Pro671 of human NUP98. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human histone H2A.Z. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
The Nucleus and Nuclear Envelope-Associated Marker Proteins Antibody Sampler Kit contains a variety of antibodies directed against established nuclear proteins (1). Histone H3 and histone H2A.Z are histone family members and components of nucleosomes, the primary building block of chromatin made up of DNA wound around eight core histone proteins. The amino-terminal tails of core histones undergo various post-translational modifications and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (2). ESET histone methyltransferase (3) and LSD1 histone demethylase (4) are both regulators of histone methylation and are chromatin-associated. Both NUP98 (5) and lamins (6) are located within the nuclear envelope (also known as the nuclear membrane). NUP98 is a component of the nuclear pore complex. Lamin A and lamin C are fibrous proteins contributing to nuclear structural and transcriptional regulation. Finally, fibrillarin (7) is located in fibrillar regions and Cajal bodies of nucleoli, where it functions to regulate RNA transcription and pre-rRNA processing.
- Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
- Jin, J. et al. (2005) Trends Biochem Sci 30, 680-7.
- Yang, L. et al. (2002) Oncogene 21, 148-52.
- Shi, Y. et al. (2004) Cell 119, 941-53.
- Fontoura, B.M. et al. (1999) J Cell Biol 144, 1097-112.
- Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
- Tollervey, D. et al. (1993) Cell 72, 443-57.
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* Product-specific protocol.
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 4499 Histone H3 (D1H2) XP® Rabbit mAb
- 2718 Histone H2A.Z Antibody
- 2196 ESET (C1C12) Rabbit mAb
- 2184 LSD1 (C69G12) Rabbit mAb
- 2639 Fibrillarin (C13C3) Rabbit mAb
- 2598 NUP98 (C39A3) Rabbit mAb
- 4777 Lamin A/C (4C11) Mouse mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.