Product Pathways - Neuroscience
Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb #12793
|12793S||100 µl (10 western blots)||---||In Stock||---|
|12793||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||52||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
* Product-specific protocol.
Specificity / Sensitivity
Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb recognizes endogenous levels of total glutamate dehydrogenase 1 and 2 proteins. Species cross-reactivity for IHC-P and IF-IC is in human only.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro190 of human glutamate dehydrogenase 1 protein.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human glutamate dehydrogenase 1 (hGluD1; +) or glutamate dehydrogenase 2 (hGluD2; +), using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb.
Western blot analysis of extracts from various cell lines and tissues using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb.
Confocal immunofluorescent analysis of A172 cells using Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Glutamate dehydrogenase is a mitochondrial enzyme that catalyzes the oxidative deamination of glutamate to α-ketoglutarate through association with the cofactor nicotinamide adenine dinucleotide phosphate (1). Glutamate dehydrogenase is highly expressed in various tissues such as the liver, brain, kidney, heart, pancreas, ovaries, and testis. Two isoforms produced by two distinct genes are found in mammalian tissues. The GLUD1 gene is ubiquitously expressed (2), while the GLUD2 gene is specifically expressed in testicular tissues and astrocytes (3,4). Glutamate dehydrogenase links glutamate to the Krebs cycle, thereby playing a critical role in the regulation of energy homeostasis. Research studies have shown that changes in glutamate dehydrogenase activity in pancreatic β-cells can cause a hyperinsulinism syndrome (5).
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