Product Pathways - Protein Stability
K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb (HRP Conjugate) #12805
|12805S||100 µl (10 western blots)||---||In Stock||---|
|12805||carrier free and custom formulation / quantity||email request|
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|W||1:1000||All Species Expected||Endogenous||Rabbit IgG|
Applications Key: W=Western Blotting
Specificity / Sensitivity
K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb (HRP Conjugate) detects polyubiquitin chains formed by Lys48 residue linkage. This antibody does not react with monoubiquitin or polyubiquitin chains formed by specific linkage to a different lysine residue.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the Lys48 branch of the human diubiquitin chain.
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb #8081.
Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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