Product Pathways - Chromatin Regulation / Epigenetics
SSU72 (D3I2D) Rabbit mAb #12816
|12816S||100 µl (10 western blots)||---||In Stock||---|
|12816||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||27||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Hamster, Chicken, Bovine, Dog, Pig, Guinea Pig.
Specificity / Sensitivity
SSU72 (D3I2D) Rabbit mAb recognizes endogenous levels of total SSU72 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro93 of human SSU72 protein.
Western blot analysis of extracts from various cell lines using SSU72 (D3I2D) Rabbit mAb.
Immunoprecipitation of SSU72 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SSU72 (D3I2D) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SSU72 (D3I2D) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.
SSU72 is a protein phosphatase originally identified through its interaction with TFIIB as a factor required for transcription and RNA processing (1,2). While SSU72 has been known as a phosphatase of the RNA polymerase II carboxy-terminal domain at Ser5 of the heptapeptide repeat, additional studies suggest a role for SSU72 as a phosphatase for Ser7 of the heptapeptide repeat as well (3-9). The activities of SSU72 are thought to play a critical role in coupling transcription with pre-mRNA 3’ end processing (10-12). SSU72 is required for gene looping that connects the gene promoter with the terminator region, leading to proper initiation of transcription and enforcement of transcriptional directionality on promoters that may other wise act as bidirectional promoters (13-14). In addition to a role in transcription, SSU72 acts as a cohesin binding protein that regulates cohesion between sister chromatid arms during mitosis or meiosis (15).
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