Product Pathways - Neuroscience
Plexin Isoform Antibody Sampler Kit #12823
|12823S||1 Kit (4 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Plexin A1 Antibody #3813||40 µl||W, IP, IHC-P||H, R||M||211||Rabbit|
|Plexin A2 (D42B5) Rabbit mAb #5658||40 µl||W, IP||H, M, R||212||Rabbit IgG|
|Plexin A3 (D2G12) Rabbit mAb #5512||40 µl||W, IP||H||207||Rabbit IgG|
|Plexin A4 (C5D1) Rabbit mAb #3816||40 µl||W, IP||M, R||H||212||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, R=Rat, M=Mouse
Western blot analysis of extracts from fetal rat brain and K562 cells using Plexin A1 Antibody #3813.
Western blot analysis of extracts from mouse brain and rat brain tissues using Plexin A4 (C5D1) Rabbit mAb #3816.
Western blot analysis of extracts from human cerebellum, A204 and MCF-7 cells using Plexin A3 (D2G12) Rabbit mAb #5512.
The Plexin Isoform Antibody Sampler Kit provides an economical means of evaluating Plexin A1, A2, A3 and A4 protein expression. The kit contains enough primary antibody to perform four western miniblot experiments per primary antibody.
Specificity / Sensitivity
Plexin A1 Antibody detects endogenous levels of total Plexin A1 protein. Plexin A2 (D42B5) Rabbit mAb detects endogenous levels of total Plexin A2 protein and may cross-react with unidentified proteins at various molecular weights. Plexin A3 (D2G12) Rabbit mAb detects endogenous levels of total Plexin A3 protein and may cross-react with other family members. Plexin A4 (C5D1) Rabbit mAb detects endogenous levels of total Plexin A4 protein.
Source / Purification
Polyclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Gln1875 of human Plexin A1 protein and purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly185 of human Plexin A2 protein, or Val440 of human Plexin A3 protein, or with a GST-fusion protein corresponding to residues from the human sequence of Plexin A4 protein.
Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3). Sema6A is a ligand for Plexin A2. Both Sema6A and Plexin A2 knock-out mice have a granule cell migration defect, where cells remain in the molecular layer. Furthermore, Plexin A2 also controls nucleus-centrosome coupling that modulates cell migration (4). Both Plexin A3 and A4 mediate the responses to class 3 semaphorins in sensory and sympathetic neurons. In particular, Plexin A4 is responsible for signaling of Sema 3A via neuropilin-1, while Plexin A3 is responsible for signaling of Sema 3F via neuropilin-2 (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.