Product Pathways - Tag Antibodies
Cre Recombinase (D3U7F) Rabbit mAb #12830
|12830S||100 µl (10 western blots)||---||In Stock||---|
|12830||carrier free and custom formulation / quantity||email request|
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|W||1:1000||All Species Expected||Transfected Only||37||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Cre Recombinase (D3U7F) Rabbit mAb recognizes transfected levels of total Cre recombinase protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly103 of bacteriophage-P1 Cre recombinase protein.
Western blot analysis of extracts from 293T cells, mock-transfected (-) or transfected with a construct expressing Cre recombinase (+), using Cre Recombinase (D3U7F) Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).
Confocal immunofluorescent analysis of ER-Cre-MEF cells, untreated (left) or treated with 4OH-Tamoxifen (200 ng/ml; center), and Cre-transfected 293 cells (right), using Cre Recombinase (D3U7F) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). The ER-Cre-MEF cells were kindly provided by Dr. Mark Bedford (M.D. Anderson Cancer Center, TX).
Flow cytometric analysis of COS-7 cells, untransfected (blue) or transfected with Cre recombinase (green), using Cre Recombinase (D3U7F) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34 base pair loxP sites. This reaction can be carried out in vitro, indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of in vitro and in vivo applications including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).
- Abremski, K. et al. (1983) Cell 32, 1301-11.
- Sternberg, N. et al. (1981) Cold Spring Harb Symp Quant Biol 45 Pt 1, 297-309.
- Abremski, K. and Hoess, R. (1984) J Biol Chem 259, 1509-14.
- Qin, M. et al. (1994) Proc Natl Acad Sci U S A 91, 1706-10.
- Lakso, M. et al. (1992) Proc Natl Acad Sci U S A 89, 6232-6.
- Guo, F. et al. (1997) Nature 389, 40-6.
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