Product Pathways - Growth Factors/Cytokines
IFN-γ (D3H2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #12942
|12942S||100 µl (50 tests)||---||In Stock||---|
|12942||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|F||1:50||Human||Endogenous||17, 19, 23||Rabbit IgG|
Applications Key: F=Flow Cytometry
Specificity / Sensitivity
IFN-γ (D3H2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total IFN-γ protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human IFN-γ protein.
Flow cytometric analysis of human peripheral blood cells, untreated (left) or treated with TPA #4174 (40 nM, 4 hr), Ionomycin, Calcium Salt #9995 (2 µM, 4 hr), and Brefeldin A #9972 (1 µg/ml, last 3 hr of stimulation) (right), using a CD3 antibody and IFN-γ (D3H2) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate). Analysis was performed on gated lymphocyte cells.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated IFN-γ (D3H2) XP® Rabbit mAb #8455.
IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes the cell mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.