Product Pathways - Apoptosis
Cytochrome c (D18C7) Rabbit mAb (HRP Conjugate) #12959
|12959S||100 µl (10 western blots)||---||In Stock||---|
|12959||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||14||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Bovine, Pig.
Specificity / Sensitivity
Cytochrome c (D18C7) Rabbit mAb (HRP Conjugate) recognizes endogenous levels of total cytochrome c protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro72 of human cytochrome c protein.
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cytochrome c (D18C7) Rabbit mAb #11940.
Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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