Product Pathways - Translational Control
SMN1 (2F1) Mouse mAb #12976
|12976S||100 µl (10 western blots)||---||In Stock||---|
|12976||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||35||Mouse IgG1|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
SMN1 (2F1) Mouse mAb recognizes endogenous levels of total SMN1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human SMN1 protein.
Western blot analysis of extracts from various cell lines using SMN1 (2F1) Mouse mAb.
Immunoprecipitation of SMN1 from Hep G2 cell extracts using Mouse (G3A1) mAb IgG1 Isotype control #5415 (lane 2) or SMN1 (2F1) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SMN1 (2F1) Mouse mAb.
Confocal immunofluorescent analysis of HeLa cells using SMN1 (2F1) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Survival of Motor Neuron 1 (SMN1) is essential for the maturation of small nuclear ribonucleoproteins (snRNPs) (1,2). SMN1 plays a role in the assembly of spliceosomal snRNPs in the cytoplasm, together with the Gemin proteins, and may also participate in the transport of snRNPs into the nucleus (3-6). SMN1 also participates in the maturation and turnover of snRNPs in nuclear foci Gemini bodies (gems) (7). In addition to the maturation of spliceosomal snRNPs, SMN1 has also been proposed to directly regulate pre-mRNA splicing (8). Researchers have found mutations and deletions of the SMN1 gene are found in 95% of Spinal Muscular Atrophy (SMA) neuromuscular disorder cases (1,9).
- Nlend Nlend, R. et al. RNA Biol 7, 430-40.
- Pellizzoni, L. et al. (2002) Science 298, 1775-9.
- Liu, Q. et al. (1997) Cell 90, 1013-21.
- Meister, G. et al. (2001) Nat Cell Biol 3, 945-9.
- Narayanan, U. et al. (2002) Hum Mol Genet 11, 1785-95.
- Huber, J. et al. (1998) EMBO J 17, 4114-26.
- Hebert, M.D. et al. (2001) Genes Dev 15, 2720-9.
- Pellizzoni, L. et al. (1998) Cell 95, 615-24.
- Wirth, B. (2000) Hum Mutat 15, 228-37.
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