Product Pathways - Cell Cycle / Checkpoint
MSH6 (3E1) Mouse mAb #12988
|12988S||100 µl (10 western blots)||---||In Stock||---|
|12988||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
* Product-specific protocol.
Specificity / Sensitivity
MSH6 (3E1) Mouse mAb recognizes endogenous levels of total MSH6 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human MSH6 protein expressed in E.coli.
Western blot analysis of extracts from various cell lines using MSH6 (3E1) Mouse mAb.
Western blot analysis of extracts from cell lines with varied expression of MSH6, using MSH6 (3E1) Mouse mAb (upper), MSH6 (D60G2) XP® Rabbit mAb #5424 (middle), or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Immunohistochemical analysis of paraffin-embedded colorectal adenocarcinoma using MSH6 (3E1) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded non-small cell lung carcinoma using MSH6 (3E1) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded cell pellets, 293T (left) and HCT-15 (right) using MSH6 (3E1) Mouse mAb.
Confocal immunofluorescent analysis of 293T (left) and HCT-15 (right) cells, using MSH6 (3E1) Mouse mAb (green) and β-Actin (D6A8) Rabbit mAb #8457 (red).
The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 5424 MSH6 (D60G2) XP® Rabbit mAb
- 2017 MSH2 (D24B5) XP® Rabbit mAb
- 3515 MLH1 (4C9C7) Mouse mAb
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
- 5425 Normal Goat Serum
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 8059 SignalStain® DAB Substrate Kit
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Tween® is a registered trademark of ICI Americas, Inc.
SignalStain® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.