Product Pathways - Growth Factors/Cytokines
IFN-γ (D3H2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #13026
|13026S||100 µl (50 tests)||---||In Stock||---|
|13026||carrier free and custom formulation / quantity||email request|
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|F||1:50||Human||Endogenous||17, 19, 23||Rabbit IgG|
Applications Key: F=Flow Cytometry
Specificity / Sensitivity
IFN-γ (D3H2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total IFN-γ protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human IFN-γ protein.
Flow cytometric analysis of human peripheral blood cells, untreated (left) or treated with TPA #4174 (40 nM, 4 hr), Ionomycin, Calcium Salt #9995 (2 µM, 4 hr), and Brefeldin A #9972 (1 µg/ml, last 3 hr of stimulation) (right), using a CD3 antibody and IFN-γ (D3H2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate). Analysis was performed on cells in the lymphocyte gate.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated IFN-γ (D3H2) XP® Rabbit mAb #8455.
IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes the cell mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.