Product Pathways - Metabolism
PFKFB2 (D5I5F) Rabbit mAb #13029
|13029S||100 µl (10 western blots)||---||In Stock||---|
|13029||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||55||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
* Product-specific protocol.
Specificity / Sensitivity
PFKFB2 (D5I5F) Rabbit mAb recognizes endogenous levels of total PFKFB2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val82 within the 6-phosphofructo-2-kinase domain of human PFKFB2 protein.
Western blot analysis of extracts from LNCaP, Hep G2, and MCF7 cells using PFKFB2 (D5I5F) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of PFKFB2 from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PFKFB2 (D5I5F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PFKFB2 (D5I5F) Rabbit mAb. An anti-rabbit IgG light chain antibody was used as the secondary antibody.
Confocal immunofluorescent analysis of LNCaP (high expression, left) and HepG2 (low expression, right) using PFKFB2 (D5I5F) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). Research studies indicate that amino acids activate PFKFB2 through Akt-dependent phosphorylation at Ser483 on PFKFB2 (3). In addition, androgen increases the expression of PFKFB2 in prostate cancer cells (4).
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