Product Pathways - Cytoskeletal Signaling
Rab25 (D4P6P) XP® Rabbit mAb #13048
|13048S||100 µl (10 western blots)||---||In Stock||---|
|13048P||40 µl (4 western blots)||---||In Stock||---|
|13048||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Rab25 (D4P6P) XP® Rabbit mAb recognizes endogenous levels of total Rab25 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rab25 protein.
Western blot analysis of extracts from various cell lines using Rab25 (D4P6P) XP® Rabbit mAb.
Western blot analysis of extracts from MCF7 (positive) and HeLa (negative) cells using Rab25 (D4P6P) XP® Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457 as a loading control.
Immunoprecipitation of Rab25 from ZR-75-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Rab25 (D4P6P) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Rab25 (D4P6P) XP® Rabbit mAb.
Rab11a, Rab11b, and Rab25 are members of the Rab11 subfamily of small Ras-like GTPases. Unlike universally expressed Rab11, typical Rab25 expression appears to be limited to gastrointestinal mucosa, kidney, and lung (1). Rab25 can associate with apical recycling vesicles to help regulate apical vesicle trafficking (2,3). Research studies indicate that atypical Rab25 expression can be associated with various forms of cancer. Increased Rab25 expression is associated with aggressive growth in ovarian and breast cancer, where Rab25 may inhibit apoptosis and promote cancer cell proliferation and invasion through regulation of vesicle transport and cellular motility (4-7). Interaction between Rab25 and β1 integrin promotes vesicle-mediated transport of integrin to pseudopodial tip membranes, fostering the persistent invasion of tumor cells (8). Conversely, the reported loss of Rab25 expression in a number of breast cancer cases has an unclear effect on cancer pathogenesis (9).
- Goldenring, J.R. et al. (1993) J Biol Chem 268, 18419-22.
- Casanova, J.E. et al. (1999) Mol Biol Cell 10, 47-61.
- Wang, X. et al. (2000) J Biol Chem 275, 29138-46.
- Cheng, K.W. et al. (2004) Nat Med 10, 1251-6.
- Cheng, K.W. et al. (2005) Cancer Res 65, 2516-9.
- Chia, W.J. and Tang, B.L. (2009) Biochim Biophys Acta 1795, 110-6.
- Tang, B.L. and Ng, E.L. (2009) Cell Motil Cytoskeleton 66, 365-70.
- Caswell, P.T. et al. (2007) Dev Cell 13, 496-510.
- Cheng, J.M. et al. (2010) Int J Cancer 126, 2799-812.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 4314 Rab25 Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 13081 Rab25 (D3H4N) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.