Product Pathways - Translational Control
Symplekin (D5Y3T) Rabbit mAb #13071
|13071S||100 µl (10 western blots)||---||In Stock||---|
|13071||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||141||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Dog, Horse.
Specificity / Sensitivity
Symplekin (D5Y3T) Rabbit mAb recognizes endogenous levels of total symplekin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human symplekin protein.
Western blot analysis of extracts from various cell lines using Symplekin (D5Y3T) Rabbit mAb.
Symplekin is necessary for 3’-end cleavage and polyadenylation of mRNAs, histone 3’-end processing and polyadenylation in the cytoplasm (1-5). It is thought to act as a scaffolding protein that brings together factors involved in mRNA 3’-end processing (1,2). Symplekin also plays a role in transcription initiation and termination by RNA polymerase II (RNAPII) through bridging the interaction between the polyadenylation machinery and RNAPII (3,6,7). In addition to its role in mRNA 3’-end processing, research studies have shown that symplekin localizes at epithelial cell tight junctions where it may help to regulate tight junction assembly, thereby maintaining the integrity of the epithelial monolayer and cell polarity (8,9).
- Takagaki, Y. and Manley, J.L. (2000) Mol Cell Biol 20, 1515-25.
- Mandel, C.R. et al. (2008) Cell Mol Life Sci 65, 1099-122.
- He, X. et al. (2003) Genes Dev 17, 1030-42.
- Dominski, Z. and Marzluff, W.F. (2007) Gene 396, 373-90.
- Barnard, D.C. et al. (2004) Cell 119, 641-51.
- Calvo, O. and Manley, J.L. (2003) Genes Dev 17, 1321-7.
- Moore, M.J. and Proudfoot, N.J. (2009) Cell 136, 688-700.
- Keon, B.H. et al. (1996) J Cell Biol 134, 1003-18.
- Chang, H. et al. (2012) Histochem Cell Biol 137, 319-27.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.