Product Pathways - Chromatin Regulation / Epigenetics
WDR5 (D3X5B) Rabbit mAb #13073
|13073S||100 µl (10 western blots)||---||In Stock||---|
|13073||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||37||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Bovine.
Specificity / Sensitivity
WDR5 (D3X5B) Rabbit mAb recognizes endogenous levels of total WDR5 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro30 of human WDR5 protein.
The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30, which are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6).
WDR5 is a core subunit of all SET1/MLL histone methyltransferase complexes and is required for proper complex assembly and histone methyltransferase activity (7). It functions as an effector of histone H3 Lys4 methylation by recruiting SET1/MLL complexes to target loci and presenting the histone H3 amino-terminal tail for methylation (8). WDR5 contains a classical, seven-bladed WD40 propeller domain with a central cavity that binds to histone H3 Arg2 when symmetrically di-methylated (H3Arg2Me2-S) by arginine methyltransferases PRMT5 and PRMT7 (8). WDR5 binding to H3Arg2Me2-S results in increased recruitment of SET1/MLL complexes and methylation of histone H3 Lys4 at gene promoters and distal regulatory sites. In contrast, asymmetric di-methylation of histone H3 Arg2 (H3Arg2Me2-A) by PRMT6 reduces WDR5 binding and results in decreased recruitment of SET1/MLL complexes and reduced histone H3 Lys4 methylation (8). Interestingly, the H3Arg2Me2-S binding pocket of WDR5 also interacts with the SET domains of SET1/MLL proteins with comparable affinity, setting up a potential competition for WDR5 binding that may act to regulate SET1/MLL recruitment and subsequent H3 Lys4 methylation (9-11). WDR5 is also a core subunit of the ATAC and MOF-NSL histone acetyltransferase complexes and the CHD8 chromatin-remodeling complex (12-14).
- Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
- Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.
- Migliori, V. et al. (2012) Epigenetics 7, 815-22.
- Migliori, V. et al. (2012) Nat Struct Mol Biol 19, 136-44.
- Song, J.J. and Kingston, R.E. (2008) J Biol Chem 283, 35258-64.
- Patel, A. et al. (2008) J Biol Chem 283, 32162-75.
- Zhang, P. et al. (2012) Nucleic Acids Res 40, 4237-46.
- Wang, Y.L. et al. (2008) J Biol Chem 283, 33808-15.
- Cai, Y. et al. (2010) J Biol Chem 285, 4268-72.
- Thompson, B.A. et al. (2008) Mol Cell Biol 28, 3894-904.
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