Product Pathways - Cell Cycle / Checkpoint
Wee1 (D10D2) Rabbit mAb #13084
|13084S||100 µl (10 western blots)||---||In Stock||---|
|13084||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry
Specificity / Sensitivity
Wee1 (D10D2) Rabbit mAb recognizes endogenous levels of total Wee1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Wee1 protein.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Wee1 (D10D2) Rabbit mAb.
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Geldanamycin #9843 (+), using Wee1 (D10D2) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using Wee1 (D10D2) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded HT-29 (left) or PC-3 (right) cell pellets using Wee1 (D10D2) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Wee1 (D10D2) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow cytometric analysis of Jurkat cells using Wee1 (D10D2) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-8).
Wee1 is inactivated upon mitotic entry by phosphorylation at Ser53 and Ser123 by Plk1 and cdc2, followed by β-TrCP-mediated ubiquitination and degradation (1,9,10).
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- Parker, L.L. et al. (1995) Proc Natl Acad Sci U S A 92, 9638-42.
- Watanabe, N. et al. (2004) Proc Natl Acad Sci U S A 101, 4419-24.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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