Product Pathways - TGF-beta/Smad Signaling
RUNX3/AML2 (D9K6L) Mouse mAb #13089
|13089S||100 µl (10 western blots)||---||In Stock||---|
|13089||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||43-48||Mouse IgG2b|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
RUNX3/AML2 (D9K6L) Mouse mAb recognizes endogenous levels of total RUNX3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Gly217 of human Runx3 protein.
Western blot analysis of extracts from SW620, HT-1080, and MCF7 cells using RUNX3/AML2 (D9K6L) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Runt-related transcription factor 3 (RUNX3, AML2), a member of the Runt family of transcription factors, plays an important role in the suppression of gastric epithelium cell proliferation (1), dorsal root ganglia neurogenesis (2), and T cell differentiation (3,4). RUNX3 is also involved in caspase-3-dependent apoptosis (5). Protein complexes containing RUNX3 and various transcription factors, such as Smads or β-catenin/TCF4, have tumor suppressor activity and regulate downstream target gene transcription (6,7). While typically localized to the nucleus, RUNX3 can be tyrosine phosphorylated and located in the cytoplasm of many cancer cells. This mislocalization of RUNX3 abolishes its tumor suppressor function and contributes to tumorigenesis (8). Research studies indicate that gene silencing or protein mislocalization inactivates RUNX3 in more than 80% of gastric cancers and other cancer types (1,9,10).
- Li, Q.L. et al. (2002) Cell 109, 113-24.
- Inoue, K. et al. (2002) Nat Neurosci 5, 946-54.
- Taniuchi, I. et al. (2002) Cell 111, 621-33.
- Woolf, E. et al. (2007) Dev Biol 303, 703-14.
- Zhai, F.X. et al. (2012) J Cancer Res Clin Oncol 138, 439-49.
- Chi, X.Z. et al. (2005) Mol Cell Biol 25, 8097-107.
- Ito, K. et al. (2008) Cancer Cell 14, 226-37.
- Goh, Y.M. et al. (2010) J Biol Chem 285, 10122-9.
- Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-87.
- Ito, K. et al. (2005) Cancer Res 65, 7743-50.
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