Product Pathways - Transcription Factors
LRF/Pokemon Antibody #13097
|13097S||100 µl (10 western blots)||---||In Stock||---|
|13097||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
LRF/Pokemon Antibody recognizes endogenous levels of total LRF (Pokemon) protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro241 of human LRF (pokemon) protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from various cell lines using LRF/Pokemon Antibody.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human LRF/Pokemon (hLRF/Pokemon-Myc/DDK; +), using LRF/Pokemon Antibody.
Western blot analysis of extracts from HeLa cells transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® LRF/Pokemon siRNA I #13350 (+) using LRF/Pokemon Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The LRF/Pokemon Antibody confirms silencing of LRF/Pokemon expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.
Zinc finger and BTB domain-containing protein 7A (LRF, Pokemon, FBI1) is a transcriptional repressor encoded by the ZBTB7A gene that belongs to the POK (POZ and Kruppel)/ZBTB (zinc finger and BTB) family (1). LRF is broadly expressed with elevated expression in a variety of cancers relative to normal tissues, including non-small cell lung cancer, breast cancer, ovarian cancer, prostate cancer, and hepatocellular carcinoma (1-8). Research studies suggest that LRF acts as an oncogene through various mechanisms including repression of the tumor suppressors ARF and Rb, and repression of the cell cycle arrest factor p21Cip1 (9-11). The LRF transcription factor plays key roles during several stages of hematopoiesis including promoting lymphoid progenitor cells to commit to B cell differentiation by repressing T cell-promoting Notch signals, and promoting cell survival during terminal erythroid differentiation through suppression of the proapoptotic factor Bim (12,13).
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- Jeon, B.N. et al. (2008) J Biol Chem 283, 33199-210.
- Choi, W.I. et al. (2009) J Biol Chem 284, 12633-44.
- Maeda, T. et al. (2007) Science 316, 860-6.
- Maeda, T. et al. (2009) Dev Cell 17, 527-40.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Tween® is a registered trademark of ICI Americas, Inc.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.