Product Pathways - Neuroscience
Vti1a Antibody #13098
|13098S||100 µl||---||In Stock||---|
|13098||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||26||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Vti1a Antibody recognizes endogenous levels of total Vti1a protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu126 of human Vti1a protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human Vti1a (hVti1a-Myc; +) , using Vti1a Antibody.
Western blot analysis of extracts from various cell lines and tissues using Vti1a Antibody.
Immunoprecipitation of Vti1a from 293T cell extracts, using Normal Rabbit IgG #2729 (lane 2) or Vti1a Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Vti1a Antibody.
Vesicle transport through interaction with t-SNAREs homolog 1 (Vti1) has two protein members, Vti1a and Vti1b. Human Vti1 was first identified as a homolog of the yeast v-SNARE Vti1p and was able to functionally rescue the phenotype of Vti1p-deficient yeast (1). The mammalian proteins Vti1a and Vti1b exhibit distinct but overlapping localization. Vti1a and Vti1b are both localized in the trans-Golgi network, with Vti1a also found in the Golgi apparatus and Vti1b in endosomes (2). Vti1 proteins have been implicated in a number of protein-protein interactions with partners such as VAMP4, syntaxin 6, syntaxin 8, syntaxin 16, and synaptobrevin (2,3,4).
Vti1a that resides on synaptic vesicles has been shown to be involved in maintaining spontaneous neurotransmitter release (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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