Product Pathways - DNA Damage
p63-α (D2K8X) XP® Rabbit mAb #13109
|13109S||100 µl (10 western blots)||---||In Stock||---|
|13109P||40 µl (4 western blots)||---||In Stock||---|
|13109||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
p63-α (D2K8X) XP® Rabbit mAb recognizes endogenous levels of total p63-α protein. This antibody will detect both the full-length isoform (TAp63-α) and the truncated δNp63-α isoform, but will not recognize the p63-β or p63-γ isoform.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p63-α protein.
Western blot analysis of extracts from ME-180 (+), HaCaT (+), and MCF7 (-) cells using p63-α (D2K8X) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Flow cytometric analysis of ME-180 (green) and MCF7 (blue) cells using p63-α (D2K8X) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Confocal immunofluorescent analysis of ME-180 (left) and MCF7 (right) cells using p63-α (D2K8X) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription initiation site that yields a 40 kDa δNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms (3,4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.