Product Pathways - Cell Cycle / Checkpoint
PCNA (D3H8P) XP® Rabbit mAb #13110
|13110S||100 µl (10 western blots)||---||In Stock||---|
|13110P||40 µl (4 western blots)||---||In Stock||---|
|13110||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||36||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
PCNA (D3H8P) XP® Rabbit mAb recognizes endogenous levels of total PCNA protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PCNA protein.
Western blot analysis of extracts from various cell lines using PCNA (D3H8P) XP® Rabbit mAb.
Immunoprecipitation of PCNA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PCNA (D3H8P) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using PCNA (D3H8P) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PCNA (D3H8P) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PCNA (D3H8P) XP® Rabbit mAb.
Flow cytometric analysis of Jurkat cells using PCNA (D3H8P) XP® Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Confocal immunofluorescent analysis of HeLa cells using PCNA (D3H8P) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Tween® is a registered trademark of ICI Americas, Inc.
SignalStain® is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.