Product Pathways - Vesicle Trafficking
CD71 (D7G9X) XP® Rabbit mAb #13113
|13113S||100 µl (10 western blots)||---||In Stock||---|
|13113P||40 µl (4 western blots)||---||In Stock||---|
|13113||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
* Product-specific protocol.
Specificity / Sensitivity
CD71 (D7G9X) XP® Rabbit mAb recognizes endogenous levels of total CD71 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu146 of human CD71 protein.
Western blot analysis of extracts from various cell lines using CD71 (D7G9X) XP® Rabbit mAb.
Immunoprecipitation of CD71 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CD71 (D7G9X) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CD71 (D7G9X) XP® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Brefeldin A #9972 (5 μg/ml, 15 min; right), using CD71 (D7G9X) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Transferrin receptor 1 (CD71, TFRC) is a type II transmembrane receptor and carrier protein responsible for the uptake of cellular iron through receptor-mediated endocytosis (1). Neutral pH at the cell surface promotes binding of the iron-binding glycoprotein transferrin (Tf) to the CD71 receptor. The receptor-ligand complex enters the cell through receptor-mediated endocytosis and is internalized into an endosome. Relatively lower endosomal pH leads to a change in the local charge environment surrounding the iron-transferrin binding site and results in the release of iron (2). The receptor-ligand complex is recycled to the cell surface where transferrin dissociates from the CD71 receptor (2). Ubiquitously expressed transferrin receptor is continuously recycled and undergoes clathrin-mediated endocytosis regardless of ligand binding state. The interaction between receptor and ligand has been studied in detail. The helical domain of CD71 directly interacts with the transferrin C-lobe and induces a conformation change in Tf to facilitate the transport process (3). Interaction between the receptor CD71 and transferrin is mediated by the membrane protein hemochromatosis (HFE). HFE binds the α-helical domain of CD71, blocking formation of the CD71-transferrin complex and inhibiting iron uptake (4,5). In addition to binding transferrin, CD71 also interacts with H-ferritin at the cell surface and transports this intracellular iron storage protein to cellular endosomes and lysosomes (6). Additional studies indicate that the transferrin receptor is an evolutionarily conserved receptor for a number or arenaviruses and at least one retrovirus (7,8). Aberrant expression of CD71 is seen in a number of cancers, including thyroid carcinomas, lymphomas, and T-lineage leukemias, suggesting a possible therapeutic role for targeted inhibition of the transferrin receptor (9,10).
- Ponka, P. and Lok, C.N. (1999) Int J Biochem Cell Biol 31, 1111-37.
- Bali, P.K. et al. (1991) Biochemistry 30, 324-8.
- Cheng, Y. et al. (2004) Cell 116, 565-76.
- Bennett, M.J. et al. (2000) Nature 403, 46-53.
- Feder, J.N. et al. (1998) Proc Natl Acad Sci U S A 95, 1472-7.
- Li, L. et al. (2010) Proc Natl Acad Sci U S A 107, 3505-10.
- Radoshitzky, S.R. et al. (2007) Nature 446, 92-6.
- Coffin, J.M. (2013) PLoS Biol 11, e1001574.
- Magro, G. et al. (2011) Thyroid 21, 267-77.
- Rodríguez, J.A. et al. (2011) Leuk Lymphoma 52, 2169-78.
- Beardmore, J. et al. (1987) J Cell Sci 87 ( Pt 4), 495-506.
- Zuk, P.A. and Elferink, L.A. (2000) J Biol Chem 275, 26754-64.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 13208 CD71 (D7S5Z) Rabbit mAb
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Tween® is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.