Product Pathways - Lymphocyte Signaling
iNOS (D6B6S) Rabbit mAb #13120
|13120S||100 µl (10 western blots)||---||In Stock||---|
|13120||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
iNOS (D6B6S) Rabbit mAb recognizes endogenous levels of total iNOS protein. This antibody does not cross-react with other NOS proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1133 of mouse iNOS protein.
Western blot analysis of extracts from Raw 264.7 cells, untreated (-) or LPS-treated (1 μg/ml, 8 hr; +), using iNOS (D6B6S) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Flow cytometric analysis of Raw309.CR.1 cells, untreated (blue) or treated with LPS (1ug/ml for 16 hours) (green), using iNOS (D6B6S) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 fragment (Alexa Fluor 488 conjugate) #4412 was used as a secondary Ab.
Confocal immunofluorescent analysis of Raw 264.7 cells, untreated (left) or treated with LPS (1 μg/ml, 16 hr; right), using iNOS (D6B6S) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).
Nitric oxide catalyzed by iNOS is involved in host defense against protozoa, bacteria, fungi and viruses. Unlike constitutively expressed eNOS and nNos, iNOS is not usually expressed in quiescent cells. iNOS is transcriptionally induced in response to bacterial endotoxins, such as LPS and proinflammatory cytokines, in macrophages and various other cell types. Transcription factors involved in iNOS transcription include NF-κB, AP-1 and STAT. Different signaling pathways either promote (Jak1/2, PKC, c-Raf, p38 MAP kinase and p44/42 MAP kinase) or inhibit (PI3 kinase) iNOS expression depending on stimulus and cell type (4).
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