Product Pathways - Ca / cAMP / Lipid Signaling
S100A6 (D3H3W) Rabbit mAb #13162
|13162S||100 µl (10 western blots)||---||In Stock||---|
|13162||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||10||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
S100A6 (D3H3W) Rabbit mAb recognizes endogenous levels of total S100A6 protein. This antibody also cross-reacts with unidentified proteins at 40 kDa and 60 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp65 of human S100A6 protein.
Western blot analysis of extracts from various cell lines using S100A6 (D3H3W) Rabbit mAb.
Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated that significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development (1-4).
Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cation in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners, which suggests that each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4).
S100A6 (calcyclin) is involved in a number of cellular processes, including exocytosis (5) and cell cycle regulation (6). In addition, S100A6 interacts with a number of proteins such as SIP, GAPDH, and annexins in a calcium-dependent fashion (7,8). Research studies demonstrate that a down regulation of corresponding S100A6 gene expression causes a decrease in cell proliferation (6).
- Heizmann, C.W. et al. (2002) Front Biosci 7, d1356-68.
- Donato, R. (2003) Microsc Res Tech 60, 540-51.
- Marenholz, I. et al. (2004) Biochem Biophys Res Commun 322, 1111-22.
- Santamaria-Kisiel, L. et al. (2006) Biochem J 396, 201-14.
- Okazaki, K. et al. (1994) J Biol Chem 269, 6149-52.
- Breen, E.C. and Tang, K. (2003) J Cell Biochem 88, 848-54.
- Nowotny, M. et al. (2003) J Biol Chem 278, 26923-8.
- Filipek, A. and Wojda, U. (1996) Biochem J 320 ( Pt 2), 585-7.
- Bao, L. et al. (2012) FEBS J 279, 4576-88.
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