Product Pathways - Vesicle Trafficking
Golgin-97 (D8P2K) Rabbit mAb #13192
|13192S||100 µl (10 western blots)||---||In Stock||---|
|13192||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||97||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Golgin-97 (D8P2K) Rabbit mAb recognizes endogenous levels of total golgin-97 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu663 of human golgin-97 protein.
Western blot analysis of extracts from SK-MEL-5, HeLa, and C2C12 cells using Golgin-97 (D8P2K) Rabbit mAb.
Immunoprecipitation of Golgin-97 from SK-MEL-5 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Golgin-97 (D8P2K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Golgin-97 (D8P2K) Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells using Golgin-97 (D8P2K) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
The Golgi-associated protein golgin A1 (GOLGA1, golgin-97) was first isolated as a Golgi complex autoantigen associated with the autoimmune disorder Sjogren's syndrome (1). The golgin-97 protein contains a carboxy-terminal GRIP domain and is a commonly used trans-Golgi network (TGN) marker. All four known mammalian GRIP domain-containing proteins (golgin-97, golgin-245, GCC88 and GCC185) are found in the TGN, share extensive alpha-helical structure, and form homodimers (2). While all four golgin proteins localize to the TGN, they exhibit different membrane-binding abilities and are found in distinct TGN regions (3). Golgin-97 and golgin-245 are targeted to the trans-Golgi network through an interaction between their GRIP domains and the Arl1 protein switch II region (4). Overexpression studies and siRNA assays with GRIP domain-containing proteins suggest that these proteins help to maintain trans-Golgi network integrity and function by controlling localization of TGN resident proteins (5). By using a Shiga toxin B fragment (STxB)-based in vitro transport assay and an E-cadherin transport model system, golgin-97 and its effector Arl1-GTP were shown to play a role in trans-Golgi endosomal trafficking (6,7). Research studies also suggest that golgin-97 may play a role in poxvirus morphogenesis and maturation (8,9).
- Griffith, K.J. et al. (1997) Arthritis Rheum 40, 1693-702.
- Luke, M.R. et al. (2005) Biochem J 388, 835-41.
- Derby, M.C. et al. (2004) J Cell Sci 117, 5865-74.
- Lu, L. and Hong, W. (2003) Mol Biol Cell 14, 3767-81.
- Yoshino, A. et al. (2003) J Cell Sci 116, 4441-54.
- Lu, L. et al. (2004) Mol Biol Cell 15, 4426-43.
- Lock, J.G. et al. (2005) Traffic 6, 1142-56.
- Alzhanova, D. and Hruby, D.E. (2006) J Virol 80, 11520-7.
- Alzhanova, D. and Hruby, D.E. (2007) Virology 362, 421-7.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Tween® is a registered trademark of ICI Americas, Inc.
DyLight™ is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.