Product Pathways - Nuclear Receptor Signaling
CRABP1 (D5W9A) Rabbit mAb #13206
|13206S||100 µl (10 western blots)||---||In Stock||---|
|13206||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||15||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Rat, Chicken, Bovine.
Specificity / Sensitivity
CRABP1 (D5W9A) Rabbit mAb recognizes endogenous levels of total CRABP1 protein. This antibody does not cross-react with other intracellular lipid-binding protein family members, CRBP1 and CRBP2.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CRABP1 protein.
Western blot analysis of extracts from various cell lines and tissues using CRABP1 (D5W9A) Rabbit mAb.
Western blot analysis of extracts from SK-N-AS (positive) and RKO (negative) cells using CRABP1 (D5W9A) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with Myc/DDK-tagged constructs expressing full-length human CRABP1 (hCRABP1-Myc/DDK; +), full-length human CRABP2 (hCRABP2-Myc/DDK; +); full-length human CRBP1 (hCRBP1-Myc/DDK; +), full-length human CRBP2 (hCRBP2-Myc/DDK; +), and full-length human FABP4 (hFABP4-Myc/DDK; +), using CRABP1 (D5W9A) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).
Vitamin A gives rise to multiple species of biologically active lipophilic metabolites, known as retinoids, which play a critical role in numerous physiological processes such as vision and embryonic development. Intracellularly, all-trans retinoic acid is bound with high affinity to either cellular retinoic acid-binding protein 1 (CRABP1) or cellular retinoic acid-binding protein 2 (CRABP2), which aids in its solubilization within the aqueous cytosolic compartment. Belonging to the intracellular lipid-binding protein family (iLBP), the human CRABPs are 74% identical at the protein level and each CRABP is highly conserved across multiple species. Research studies have shown that knockout of Crabp1 is not lethal but results in defects in limb development (1), suggesting that CRABP1 plays a role in establishing retinoic acid concentration gradients in the developing limb bud. Although it remains unclear how CRABP1 may regulate the formation of retinoic acid gradients in vivo, research studies have suggested that CRABP1 can enhance the activities of intracellular retinoic acid-metabolizing enzymes, thus blunting cellular responses to retinoic acid (2-4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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