Product Pathways - Translational Control
DCP1B (D2P9W) Rabbit mAb #13233
|13233S||100 µl (10 western blots)||---||In Stock||---|
|13233||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||75||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
DCP1B (D2P9W) Rabbit mAb recognizes endogenous levels of total DCP1B protein. This antibody may cross-react with DCP1A.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val202 of human DCP1B protein.
Western blot analysis of extracts from Hep G2 and MCF7 cells using DCP1B (D2P9W) Rabbit mAb.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing either Myc/DDK-tagged full-length human DCP1A (hDCP1A-Myc/DDK; +) or Myc/DDK-tagged full-length human DCP1B (hDCP1B-Myc/DDK; +), using DCP1B (D2P9W) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Immunoprecipitation of DCP1B from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DCP1B (D2P9W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DCP1B (D2P9W) Rabbit mAb.
mRNA decapping is an important process in the mRNA turnover (1). DCP1A and DCP2 were identified as two human decapping enzymes and homologs of the better-characterized S. cerevisiae enzymes. Both putative decapping enzymes interact with the regulator of nonsense transcripts 1 (UPF1) and may be recruited by UPF1 or related proteins to mRNA sequences that contain premature termination codons (1). Additional research studies demonstrate that DCP1A, DCP1B (the homolog of DCP1A) and DCP2 colocalize with decapping activation factors RCK/p54 and Lsm proteins in cytoplasmic loci (2). DCP1A, DCP1B and DCP2 are components of cytoplasmic processing (P) bodies, with hyper-phosphorylation of DCP1A during mitosis suggesting a possible mechanism of P-body regulation during the cell cycle (3,4).
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