Product Pathways - Adhesion
Adherens Junction Antibody Sampler Kit #13236
|13236S||1 Kit (5 x 40 µl)||---||In Stock||---|
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|Kit Includes||Quantity||Applications||Reactivity||Homology†||MW (kDa)||Isotype|
|Afadin Antibody #6492||40 µl||W||H, M, R, Mk||205||Rabbit|
|α-E-Catenin (23B2) Rabbit mAb #3240||40 µl||W, IP||H, M, Mk||100||Rabbit IgG|
|β-Catenin (D10A8) XP® Rabbit mAb #8480||40 µl||W, IP, IHC-P, IHC-F, IF-F, IF-IC, F, ChIP||H, M, R, Mk||Z, B, Pg, GP, Hr||92||Rabbit IgG|
|γ-Catenin Antibody #2309||40 µl||W, IP, IHC-P, IF-IC||H, M, R, Hm, Mk||83||Rabbit|
|Catenin δ-1 Antibody #4989||40 µl||W, F||H, M, R||100||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, ChIP=Chromatin IP
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, Hm=Hamster
Western blot analysis of extracts from 293, MCF-7 and C2C12 cells using γ-Catenin Antibody #2309.
Western blot analysis of extracts from various cell lines using α-E-Catenin (23B2) Rabbit mAb #3240.
Western blot analysis of extracts from MDA-MB-231 and NIH/3T3 cells using Catenin δ-1 Antibody #4989.
Western blot analysis of extracts from various cell lines using Afadin Antibody #6492.
The Adherens Junction Antibody Sampler Kit provides an economical means of detecting the protein components of adherens junctions. The kit includes enough antibody to perform four western blot experiments per primary antibody.
Specificity / Sensitivity
Each antibody in this kit detects endogenous levels of total protein for the specified target and does not cross-react with other family members unless indicated. The α-E-Catenin (23B2) Rabbit mAb may cross-react with neuronal α-N-catenin. The γ-Catenin Antibody does not cross-react with β-catenin. Based on sequence homology, the Afadin Antibody is expected to recognize all isoforms of afadin.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro574 of human afadin protein, the carboxy terminus of human γ-catenin protein, or the sequence of human catenin δ-1 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human α-E-catenin or to residues surrounding Pro714 of human β-catenin protein.
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Research studies have demonstrated that loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1).
Research studies also suggest that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4).
Afadin has two splice variants: l-afadin, which is ubiquitously expressed, and s-afadin, which is expressed predominantly in neural tissue. s-afadin is a shorter form lacking one of the three proline-rich regions found in l-afadin, as well as the carboxyl-terminal F-actin binding region (5). Human s-afadin is identical to AF-6, the ALL-1 fusion partner involved in acute myeloid leukemias (6). Recent research has also shown that afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells (7,8).
- Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25.
- Yamada, S. et al. (2005) Cell 123, 889-901.
- Drees, F. et al. (2005) Cell 123, 903-15.
- Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65.
- Mandai, K. et al. (1997) J Cell Biol 139, 517-28.
- Prasad, R. et al. (1993) Cancer Res 53, 5624-8.
- Miyata, M. et al. (2009) J Cell Sci 122, 4319-29.
- Miyata, M. et al. (2009) J Biol Chem 284, 24595-609.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Select rabbit monoclonal antibodies are developed, validated, and produced at CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and in some instances 7,429,487) from Epitomics, Inc.