Product Pathways - CD Markers
CD46 (D6N7H) Rabbit mAb #13241
|13241S||100 µl (10 western blots)||---||In Stock||---|
|13241||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||50-70||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin)
Specificity / Sensitivity
CD46 (D6N7H) Rabbit mAb recognizes endogenous levels of total CD46 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro199 of human CD46 protein.
Western blot analysis of extracts from various cell lines using CD46 (D6N7H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded HeLa (left) and CHO (right) cell pellets using CD46 (D6N7H) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded colon carcinoma using CD46 (D6N7H) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Complement Regulatory Protein; Membrane Cofactor Protein (CD46) is a type 1 membrane protein that plays an important inhibitory role in the complement system (1). CD46 exhibits a cofactor activity that promotes inactivation of C3b and C4b by serum factor 1, thereby protecting host (self) cells from complement-dependent cytotoxicity (1,2). The importance of CD46 to complement regulation is underscored by the observation that genetic loss of CD46 leads to development of atypical hemolytic-uremic syndrome (aHUS), a disease characterized by uncontrolled complement activation (2,3). In addition to its role in complement inactivation, CD46 can function as a receptor for selected bacteria and viruses (4), and is reportedly required for proper fusion of spermatozoa to the oocyte membrane during fertilization (5). CD46 is implicated in the development and/or progression of selected cancer types. For example, research studies show elevated CD46 expression in medulloblastoma tumor samples (6), while CD46 expression has been linked with poor prognosis in breast cancer (7). It has been suggested that upregulation of CD46 may serve to protect cancer cells from complement-dependent cytotoxicity, thereby evading destruction by the immune system (8,9).
- Liszewski, M.K. et al. (1991) Annu Rev Immunol 9, 431-55.
- Riley-Vargas, R.C. et al. (2004) Trends Immunol 25, 496-503.
- Noris, M. and Remuzzi, G. (2009) N Engl J Med 361, 1676-87.
- Cattaneo, R. (2004) J Virol 78, 4385-8.
- Taylor, C.T. et al. (1994) Hum Reprod 9, 907-11.
- Studebaker, A.W. et al. (2010) Neuro Oncol 12, 1034-42.
- Maciejczyk, A. et al. (2011) Appl Immunohistochem Mol Morphol 19, 540-6.
- Zhang, S. et al. (2013) FEBS Lett 587, 645-51.
- Cui, W. et al. (2012) FEBS Lett 586, 766-71.
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