Product Pathways - Metabolism
PKM2 (D78A4) XP® Rabbit mAb (Sepharose® Bead Conjugate) #13266
|13266S||400 µl (40 immunoprecipitations)||---||In Stock||---|
|13266||carrier free and custom formulation / quantity||email request|
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|IP||1:20||Human, Mouse, Rat, Monkey||Endogenous||60||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IP=Immunoprecipitation
Specificity / Sensitivity
PKM2 (D78A4) XP® Rabbit mAb (Sepharose® Bead Conjugate) detects endogenous levels of total PKM2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PKM2 protein.
Immunoprecipitation of PKM2 from 3T3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 (lane 2) and PKM2 (D78A4) XP® Rabbit mAb (Sepharose® Bead Conjugate) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PKM2 (D78A4) XP® Rabbit mAb #4053 and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127.
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose® beads. PKM2 (D78A4) XP® Rabbit mAb (Sepharose® Bead Conjugate) is useful for the immunoprecipitation of PKM2. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PKM2 (D78A4) XP® Rabbit mAb #4053.
Pyruvate kinase is a glycolytic enzyme that catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2) is an alternatively spliced variant of M1 that is expressed during embryonic development (1). Research studies found that cancer cells exclusively express PKM2 (1-3). PKM2 is shown to be essential for aerobic glycolysis in tumors, known as the Warburg effect (1). When cancer cells switch from the M2 isoform to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased (1). These cells also show decreased tumorigenicity in mouse xenografts (1). Recent studies showed that PKM2 is not essential for all tumor cells (4). In the tumor model studied, PKM2 was found to be active in the non-proliferative tumor cell population and inactive in the proliferative tumor cell population (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Sepharose® is a registered trademark of GE Healthcare.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.