Product Pathways - Protein Stability
PSMB6 (E1K9O) Rabbit mAb #13267
|13267S||100 µl (10 western blots)||---||In Stock||---|
|13267||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||22||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Zebrafish, Bovine, Dog, Pig, Horse.
Specificity / Sensitivity
PSMB6 (E1K9O) Rabbit mAb recognizes endogenous levels of total PSMB6 protein. Based upon sequence alignment, this antibody is predicted to react with precursor and mature forms of PSMB6. This antibody does not cross-react with PSMB9 but does cross-react with a 60 kDa protein of unknown origin in extracts derived from some cell lines.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys67 of human PSMB6 protein.
Western blot analysis of extracts from various cell lines using PSMB6 (E1K9O) Rabbit mAb.
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 72 hr; +), using PSMB6 (E1K9O) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMB6 (hPSMB6-Myc/DDK; +) or Myc-tagged full-length human PSMB9 (hPSMB9-Myc; +), using PSMB6 (E1K9O) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).
The 26S proteasome is a highly abundant, ~2 MDa complex that serves as the proteolytic arm of the ubiquitin-proteasome system. It consists largely of two sub-complexes, the 19S regulatory particle (RP) and the 20S catalytic core particle (CP); in many cases two RPs cap either end of a CP. The CP is made of two stacked β-rings that contain the catalytic sites, each of which is made of seven subunits (β1-7), flanked on either side by two α-rings, which are also made of seven subunits each (α1-7). Thus, the structure of the 20S CP is α1-7β1-7β1-7α1-7. The RP includes a base and a lid. The base, in part, is composed of a hexametric ring of ATPases that function to unfold the substrate and open the gate of the interlacing amino-terminal segments of the α-subunits, thus allowing entry of the unfolded substrate into the catalytic chamber. The lid is predominantly involved in specific recognition of the ubiquitin signal (1). In addition to the 19S cap, other proteins and complexes, such as proteasome activator 28 (PA28/11S), bind to the end of the 20S cylinder and activate it by facilitating opening of the gate. Furthermore, proteasome-associated DUBs and E3s can remodel substrate-anchored polyubiquitin chains, which may modulate their susceptibility to degradation (2).
The core particle exhibits three distinct enzymatic activities, each catalyzed by a separate protein subunit. The constitutively expressed PSMB5, PSMB7, and PSMB6 subunits provide chymotrypsin-like, trypsin-like, and caspase-like activities, respectively. These catalytic subunits belong to the amino-terminal nucleophile (Ntn) hydrolase family and are characterized by a single-residue active site. The catalytic β-subunits are synthesized with amino-terminal propeptides, which are removed at the final step of proteasome biogenesis to expose the catalytic threonine residues (3). In immune cells involved in antigen presentation, the constitutively expressed PSMB6, PSMB7, and PSMB5 subunits are replaced by three highly homologous, induced β-subunits to form the immunoproteasome (4,5). PSMB6 is downregulated at the protein level by IFN-γ and replaced by PSMB9 in order to remodel the proteolytic specificity of the proteasome for more appropriate immunological processing of endogenous antigens (6-8).
- Finley, D. (2009) Annu Rev Biochem 78, 477-513.
- Lee, M.J. et al. (2011) Mol Cell Proteomics 10, R110.003871.
- Murata, S. et al. (2009) Nat Rev Mol Cell Biol 10, 104-15.
- Boes, B. et al. (1994) J Exp Med 179, 901-9.
- Cardozo, C. and Kohanski, R.A. (1998) J Biol Chem 273, 16764-70.
- Akiyama, K. et al. (1994) Science 265, 1231-4.
- Akiyama, K. et al. (1994) FEBS Lett 343, 85-8.
- Gaczynska, M. et al. (1996) J Biol Chem 271, 17275-80.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 12919 PSMB5 (D1H6B) Rabbit mAb
- 13207 PSMB7 (E1L5H) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Tween® is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.