Product Pathways - Metabolism
ATPIF1 (D6P1Q) XP® Rabbit mAb #13268
|13268S||100 µl (10 western blots)||---||In Stock||---|
|13268P||40 µl (4 western blots)||---||In Stock||---|
|13268||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||12||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ATPIF1 (D6P1Q) XP® Rabbit mAb recognizes endogenous levels of total ATPIF1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala46 of human ATPIF1 protein.
Western blot analysis of extracts from various cell lines using ATPIF1 (D6P1Q) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ATPIF1 (D6P1Q) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ATPIF1 (D6P1Q) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ATPIF1 (D6P1Q) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
The ATPase inhibitor factor 1 (ATPIF1) gene encodes a mitochondrial ATPase inhibitor that limits ATP depletion when mitochondrial respiration is impaired (1). ATPIF1 becomes activated following a drop in pH, binding to β-F1-ATPase, thereby inhibiting the hydrolase activity of the H+-ATP synthase (1,2). In addition to its role as an ATP hydrolase, ATPIF1 has also been shown to play a regulatory role in cellular energy metabolism by triggering the induction of aerobic glycolysis in cancer cells resulting in their Warburg phenotype (3,4). Research studies demonstrate that the overexpression of ATPIF1 in several human carcinomas further supports its participation in oncogenesis and provides insight into the altered metabolism of cancer cells, which includes the reprogramming of energetic metabolism toward glycolysis (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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