Product Pathways - Neuroscience
Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb #13281
|13281S||100 µl (10 western blots)||---||In Stock||---|
|13281||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||70||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb recognizes endogenous levels of merlin protein only when phosphorylated at Ser518.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser518 of human merlin protein.
Western blot analysis of extracts from PC-3 cells and wild-type (wt) and Nf2 knockout (-/-) mouse embryonic fibroblasts (MEFs), untreated (-) or λ phosphatase-treated (+), using Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb (upper), Merlin (D3S3W) Rabbit mAb #12888 (middle), or Ezrin/Radixin/Moesin Antibody #3142 (lower). (MEF wt and MEF Nf2 (-/-) cells were kindly provided by Dr. Andrea McClatchey, MGH Cancer Center and Harvard Medical School, Charlestown MA).
Immunoprecipitation of Phospho-Merlin (Ser518) protein from PC-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb.
Neurofibromatosis 2 (NF2) is an autosomal dominant, inherited disorder characterized by the occurrence of vestibular schwannomas, meningiomas, and other nervous system tumors. Both the familial tumors of NF2 and equivalent sporadic tumors found in the general population are caused by inactivation of the NF2 tumor suppressor gene. Merlin (moesin, ezrin, and radixin-like protein) is the NF2 gene product, displaying striking similarity to ezrin, radixin, and moesin (ERM) proteins. Regulation of merlin (also called schwannomin) and ERM proteins involves intramolecular and intermolecular head-to-tail associations between family members (1). Merlin and ERM proteins act as linkers between the plasma membrane and the cytoskeleton, affecting cell morphology, polarity, and signal transduction (2). Merlin is phosphorylated by the Rac/Cdc42 effector p21-activated kinase (PAK) at Ser518, negatively regulating Rac (3,4).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 12896 Merlin (D6N8H) Rabbit mAb
- 12888 Merlin (D3S3W) Rabbit mAb
- 2461 Phospho-Rac1/cdc42 (Ser71) Antibody
- 2601 Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody
- 2602 PAK1 Antibody
- 2604 PAK1/2/3 Antibody
- 3141 Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody
- 3142 Ezrin/Radixin/Moesin Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Tween® is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.