Product Pathways - Protein Translation
CNOT3 (E1L9S) Rabbit mAb #13300
|13300S||100 µl (10 western blots)||---||In Stock||---|
|13300||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Mouse, Rat.
Specificity / Sensitivity
CNOT3 (E1L9S) Rabbit mAb recognizes endogenous levels of total CNOT3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu638 of human CNOT3 protein.
Western blot analysis of extracts from various cell lines using CNOT3 (E1L9S) Rabbit mAb.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CNOT3 (hCNOT3-Myc/DDK; +), using CNOT3 (E1L9S) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Immunoprecipitation of CNOT3 from PANC-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CNOT3 (E1L9S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CNOT3 (E1L9S) Rabbit mAb.
The evolutionarily conserved CCR4-NOT (CNOT) complex regulates mRNA metabolism in eukaryotic cells (1). This regulation occurs at different levels of mRNA synthesis and degradation, including transcription initiation, elongation, deadenylation, and degradation (1). Multiple components, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT6L, CNOT7, CNOT8, CNOT9, and CNOT10 have been identified in this complex (2). In addition, subunit composition of this complex has been shown to vary among different tissues (3).
Research studies indicate that CNOT3 (along with CNOT1 and CNOT2) represses early developmental transcription factor expression, helping to maintain embryonic stem (ES) cell identity in mice and humans (4). Additional studies suggest that CNOT3 plays a role in mitotic progression as it destabilizes mitotic spindle assembly protein MAD1 mRNA (5). Finally, CNOT3 appears to act as a modifier gene affecting the penetrance of mutations causing autosomal dominant retinitis pigmentosa (6) and as a tumor suppressor associated with cases of adult T-cell acute lymphoblastic leukemia (7).
- Denis, C.L. and Chen, J. (2003) Prog Nucleic Acid Res Mol Biol 73, 221-50.
- Lau, N.C. et al. (2009) Biochem J 422, 443-53.
- Chen, C. et al. (2011) Biochem Biophys Res Commun 411, 360-4.
- Zheng, X. et al. (2012) Stem Cells 30, 910-22.
- Takahashi, A. et al. (2012) Biochem Biophys Res Commun 419, 268-73.
- Venturini, G. et al. (2012) PLoS Genet 8, e1003040.
- De Keersmaecker, K. et al. (2013) Nat Genet 45, 186-90.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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