Product Pathways - Adhesion
CD102/ICAM-2 (D7P2Q) Rabbit mAb #13355
|13355S||100 µl (10 western blots)||---||In Stock||---|
|13355||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human||Endogenous||45, 55||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
CD102/ICAM-2 (D7P2Q) Rabbit mAb recognizes endogenous levels of total CD102 (ICAM-2) protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CD102 (ICAM-2) protein.
Western blot analysis of extracts from various cell lines using CD102/ICAM-2 (D7P2Q) Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).
Western blot analysis of extracts from Jurkat cells, untreated (lane 1; -), mock treated (lane 2; -), or peptide-N-glycosidase F (PGNaseF)-treated (lane 3; +), using CD102/ICAM-2 (D7P2Q) Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).
Immunoprecipitation of CD102/ICAM-2 from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CD102/ICAM-2 (D7P2Q) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CD102/ICAM-2 (D7P2Q) Rabbit mAb. A light chain specific secondary antibody was used.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CD102/ICAM-2 (D7P2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD102/ICAM-2 (D7P2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded Jurkat (high expression, left) or 293T (low expression, right) cell pellets using CD102/ICAM-2 (D7P2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human tonsil using CD102/ICAM-2 (D7P2Q) Rabbit mAb.
Flow cytometric analysis of HeLa cells (blue) and HUVEC (green) using CD102/ICAM-2 (D7P2Q) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
Confocal immunofluorescent analysis of HUVEC (high expression, left) and HeLa cells (low expression, right) using CD102/ICAM-2 (D7P2Q) Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Intercellular cell adhesion molecule-2 (CD102/ICAM-2) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. Like CD54/ICAM-1, CD102/ICAM-2 is a ligand that binds the leukocyte adhesion molecule LFA-1 (leukocyte function-associated antigen-1), which mediates intercellular interactions between immune cells and other cell types (1).
Expression of CD102/ICAM-2 has been shown to affect angiogenesis (2), cellular radioresistance (3) and anti-tumor immune response (4). Along with CD54/ICAM-1, CD102/ICAM-2 mediates T cell crawling and diapedesis across the blood-brain barrier (5), as well as T cell migration across the bronchial epithelium (6). CD102/ICAM-2 interaction with the actin cytoskeleton through α-actinin has been shown to limit the mobility on neuroblastoma cells (7), and this effect is dependent on glycosylation of CD102/ICAM-2 (8).
- Staunton, D.E. et al. (1989) Nature 339, 61-4.
- Huang, M.T. et al. (2005) Blood 106, 1636-43.
- Ishigami, T. et al. (2008) Br J Cancer 98, 1357-65.
- Hiraoka, N. et al. (2011) Gastroenterology 140, 310-21.
- Steiner, O. et al. (2010) J Immunol 185, 4846-55.
- Porter, J.C. and Hall, A. (2009) FASEB J 23, 492-502.
- Yoon, K.J. et al. (2008) PLoS One 3, e3629.
- Feduska, J.M. et al. (2013) BMC Cancer 13, 261.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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