Product Pathways - Metabolism
PHGDH Antibody #13428
|13428S||100 µl (10 western blots)||---||In Stock||---|
|13428||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||57||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
PHGDH Antibody recognizes endogenous levels of total PHGDH protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln29 of human PHGDH protein. Antibodies are purified by protein A and peptide affinity chromatography.
Mammalian cells synthesize serine de novo by diverting a portion of the glycolytic intermediate 3-phosphoglycerate into the phosphorylated pathway of serine synthesis. This shift supports anabolism by providing precursors for the biosynthesis of proteins, nucleotides, creatine, porphyrins, phospholipids, and glutathione. Phosphoglycerate dehydrogenase (PHGDH) catalyzes the first step in the serine biosynthesis pathway by converting 3-phosphoglycerate into phosphohydroxy pyruvate (1).
Research studies demonstrate that an increase in serine biosynthesis supports growth and proliferation of cancer cells (2-4), which is supported by amplification and overexpression of PHGDH in a subset of melanoma and breast cancers (5,6). Suppression of PHGDH expression in cell lines with elevated PHGDH levels causes a strong decrease in cell proliferation and inhibits tumor growth in vivo (5). Additional evidence suggests that PHGDH interacts with and stabilizes FoxM1, which promotes the proliferation, invasion, and tumorigenicity of glioma cells (7).
- Locasale, J.W. (2013) Nat Rev Cancer 13, 572-83.
- Amelio, I. et al. (2013) Oncogene, [Epub ahead of print].
- Ma, L. et al. (2013) Cell 152, 599-611.
- Maddocks, O.D. et al. (2013) Nature 493, 542-6.
- Possemato, R. et al. (2011) Nature 476, 346-50.
- Locasale, J.W. et al. (2011) Nat Genet 43, 869-74.
- Liu, J. et al. (2013) J Neurooncol 111, 245-55.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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