Product Pathways - DNA Damage
EXPAND1/MUM1 (D1Z6Y) Rabbit mAb #13437
|13437S||100 µl (10 western blots)||---||In Stock||---|
|13437||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey||Endogenous||85||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
EXPAND1/MUM1 (D1Z6Y) Rabbit mAb recognizes endogenous levels of total EXPAND1/MUM1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg692 of human EXPAND1/MUM1 protein.
Western blot analysis of extracts from various cell lines using EXPAND1/MUM1 (D1Z6Y) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human EXPAND1/MUM1 (hEXPAND1/MUM1-Myc/DDK; +) protein, using EXPAND1/MUM1 (D1Z6Y) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).
DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Melanoma associated antigen (mutated) 1 (MUM1, EXPAND1) is a PWWP-domain containing chromatin binding protein involved in maintaining chromatin architecture of interphase chromosomes. In response to DNA damage, EXPAND1/MUM1 accumulates at sites of DNA double strand breaks through direct interaction with DNA repair factor 53BP1 (1). Accumulation of EXPAND1/MUM1 at damaged DNA sites is thought to modify the structure of the chromatin and allow access to other DNA repair factors (2). 53BP1 activates the checkpoint kinase ATM and promotes DNA double strand break repair via nonhomologous end joining (NHEJ) repair pathway (3).
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