Product Pathways - Transcription Factors
Helios Antibody #13459
|13459S||100 µl (10 western blots)||---||In Stock||---|
|13459||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Helios Antibody recognizes endogenous levels of total Helios protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro424 of human Helios protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human Helios (hHelios-Myc; +), using Helios Antibody.
Immunoprecipitation of Helios from EL4 cell extracts using Normal Rabbit IgG #2729 (lane 2) or Helios Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Helios Antibody.
Helios (Ikaros family zinc finger 2, IZKF2) is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family proteins (1,2). In the hematopoietic system, Helios expression is restricted to T cells and early hematopoietic progenitors (1,2). In regulatory T cells, expression of Helios contributes to an anergic phenotype by binding to the IL-2 promoter and suppressing IL-2 transcription (3). In addition, alteration of the corresponding Helios gene IZKF2 is one hallmark of low-hypodiploid acute lymphoblastic leukemia (4).
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