Product Pathways - Cell Cycle / Checkpoint
Phospho-Cyclin B1 (Ser116) Antibody #13495
|13495S||100 µl (10 western blots)||---||In Stock||---|
|13495||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Phospho-Cyclin B1 (Ser116) recognizes endogenous levels of cyclin B1 protein only when phosphorylated at Ser116.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser116 of human cyclin B1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of HT-29 cell extracts, untreated (-) or treated with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using Phospho-Cyclin B1 (Ser116) Antibody (upper), Cyclin B1 (D5C10) XP® Rabbit mAb #12231 (middle), or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Western blot analysis of HT-29 cell extracts, untreated (AS) or synchronized in S-phase by double thymidine block (2 mM) followed by release into fresh medium for the indicated time, using Phospho-Cyclin B1 (Ser116) Antibody (upper), Cyclin B1 (D5C10) XP® Rabbit mAb #12231 (middle), or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Western blot anaylsis of HeLa cell extracts, untreated (-) or treated with Nocodazole #2190 (10 nM, 24 hr; +) using Phospho-Cyclin B1 (Ser116) Antibody (upper), Cyclin B1 (D5C10) XP® Rabbit mAb #12231 (middle), or α-Actinin (D6F6) XP® rabbit mAb #6487 (lower). Membranes were mock treated (- CIP) or Calf Intestinal Alkaline Phosphatase treated (+ CIP) after transfer.
Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).
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